Acetamide Agar (Twin Pack)

Acetamide Agar (Twin Pack) is recommended for confirmation of Pseudomonas aeruginosa in water samples.formulated as per the recommendation of Standard Methods for the Examination of Water and Wastewater. Acetamide deamination leads to the liberation of ammonia, which thereby increases the pH of the medium, leading to a subsequent colour change of the phenol red indicator from yellow orange to purplish red. Some strains require up to seven days to exhibit a positive reaction as they deaminate acrylamide slowly. However, only about 40% of apyocyanogenic strains of Pseudomonas aeruginosa exhibit a positive reaction. It is therefore, not advisable to rely on this test as the only criterion for identification.