Brilliant Green Bile Agar

for enumeration of coliform bacteria from materials of sanitary importance. The medium contains a combination of brilliant green and bile, which is highly selective for coliforms, inhibiting most of the gram-positive bacteria including lactose fermenting Clostridia and some gram-negative bacteria. Erioglaucine and basic fuchsin together form the indicator system of the medium. When the pH is neutral, colour of the medium is blue while acid production from lactose turns the medium pink and colonies appear pink to deep red depending on the pH change. It is recommended that the medium be prepared just prior to use and if the medium has to be stored, it should be kept in dark