Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Peptone Water
As low as
$44.00
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
M028S
As all purpose growth medium and as a base for carbohydrate fermentation media. It is recommended by BIS committee under the specifications IS:5887(Part I)-1976.
Your enquiry has been submitted
Please login to Post an Enquiry.
Peptone Water
Peptone Water is used as all purpose growth medium and as a base for carbohydrate fermentation medium
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptic digest of animal tissue | 20.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.
Principle And Interpretation
Peptone Water is recommended by BIS (1) for detection of Indole production by Escherichia coli. A slightly different formulation of Peptone water/ Peptone water with phenol red is recommended (2,3,4) for studying the ability of organisms to ferment a specific carbohydrate which aids in differentiation of genera and species. Peptone water with phenol red is recently recommended by ISO committee (5) to study fermentation reactions of Yersinia entrocolitica. Peptone water with pH adjusted to 8.4 is suitable for the cultivation and enrichment of Vibrio species.
Peptic digest of animal tissue provides essential nutrients. Sodium chloride maintains the osmotic balance. To detect indole production add 0.5 ml of Kovacs reagent to the tube and shake the tube gently. Appearance of a red colour indicates presence of indole.
Quality Control
Appearance Light yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium Light amber coloured clear solution
Reaction Reaction of 2.5% w/v aqueous solution at 25°C. pH: 7.4±0.2
Cultural Response
M028S: Cultural characteristics observed after an incubation at 35-37°C for 24 - 48 hours.
| Organism | Inoculum (CFU) | Growth | Indole |
|---|---|---|---|
| Cultural Response | |||
| Enterobacter aerogenes ATCC 13048 | 50-100 | luxuriant | Negative reaction, no colour development / cloudy ring |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | Positive reaction, red ring at the interface of the medium |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | Negative reaction, no colour development / cloudy ring |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | Negative reaction, no colour development / cloudy ring |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Bureau of Indian Standards IS: 5887 (Part I) 1976, reaffirmed 1986.
- Macfaddin J., 1980, Biochemical Tests for Identification of Medical Bacteria, 2nd ed., Williams and Wilkins, Baltimore.
- Finegold and Baron, 1986, Bailey and Scotts Diagnostic Microbiology, 7th ed., The C.V Mosby Co., St. Louis.
- Lennette and others (Eds), 1985, Manual of Clinical Microbiology, 4th ed. ASM, Washington D.C.
- International Organization for Standardization (ISO), 1994, Draft ISO/ DIS 10273.
| Product Name | Peptone Water |
|---|---|
| SKU | M028S |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. MacFaddin J., 1980, Biochemical Tests for Identification of Medical Bacteria, 2nd ed., Williams and Wilkins, Baltimore. 2.Finegold and Baron, 1986, Bailey and Scotts Diagnostic Microbiology, 7th ed., The C.V. Mosby Co., St. Louis. 3.Lennette and others (Eds.), 1985, Manual of Clinical Microbiology, 4th ed, ASM, Washington, D.C. 4.Shread P., Donovan T.J, and Lee J.V, (1981), Soc. Gen, Microbiol. Q., 8, 184. 5.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition. 6.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. 7.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C. 8.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination ofFoods, 5th Ed., American Public Health Association, Washington, D.C. 9.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water andWastewater, 23rd ed., APHA, Washington, D.C. 10.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C. |
| Customized Product Available | No |
Write Your Own Review
HiVeg™ Peptone Water
As low as
$40.70
Peptone Water
As low as
$40.70
Peptone Water (γ-Irradiated)
As low as
$135.30
Peptone Water w/ Phenol Red
As low as
$135.30
Alkaline Peptone Water
As low as
$44.00
Upsell Products
Peptone Water
As low as
$36.30
Andrade Peptone Water
As low as
$52.80
HiCulture™ Transport Swabs w/0.1% Peptone water
As low as
$28.60
HiCulture™ Transport Swab w/ 0.1% Peptone Water
As low as
$29.70