Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Phenol Red Broth Base
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$33.00
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SKU:
LQ173
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Intended Use
As a base for carohydrate fermentation reaction study of pure cultures of microorganisms.
Composition
| Ingredients | g/L |
|---|---|
| Proteose peptone | 10.000 |
| HM peptone B # | 1.000 |
| Sodium chloride | 5.000 |
| Phenol red | 0.018 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Directions
Label the ready to use LQ173 bottle. Inoculate 50-100 cfu sample and Incubate at specified temperature and time.
Principle And Interpretation
Phenol Red Broth Medium is formulated as per Vera (1) and is recommended to determine the fermentation reaction of carbohydrates for the differentiation of microorganisms (2,3,4). Phenol Red Broth Medium with various added carbohydrates serves as a differential medium by aiding in differentiation of various species and genera by their ability to ferment the specific carbohydrate, with the production of acid or acid and gas (5). Phenol Red Broth Base is a complete medium without added carbohydrate, which can be used with the addition of 5-10% desired carbohydrate. It is used as a negative control for studying fermentations or as a base for the addition of carbohydrates. Proteose peptone and HM peptone B serve as sources for carbon and nitrogen. Sodium chloride is the osmotic stabilizer. Phenol red is the pH indicator, which turns yellow at acidic pH.All of the Enterobacteriaceae grow well in this medium. In addition to producing a pH colour shift, the production of mixed acids, notably butyric acids, often results in a pungent, foul odour from the culture medium (6).
Type of specimen
Isolated Microorganisms from clinical and non-clinical samples.
Specimen Collection and Handling
For isolated microorganisms samples follow appropriate techniques for handling specimens as per established guidelines (7,8). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. In addition to producing a pH colour shift, the production of mixed acids, notably butyric acids, often results in a pungent, foul odour from the culture medium (2).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
AppearanceSterile Phenol red broth in bottles.
ColourRed coloured medium.
Quantity of Medium5 ml of medium in bottles.
pH7.20-7.60
Sterility CheckPasses release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours.
| Organism | Inoculum (CFU) | Growth | without carbohydrate, (Acid) | with dextrose, (Acid) |
|---|---|---|---|---|
| Citrobacter freundii ATCC 8090 | 50-100 | luxuriant | Negative reaction, no colour change | Positive reaction, yellow colour |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | Negative reaction, no colour change | Positive reaction, yellow colour |
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | luxuriant | Negative reaction, no colour change | Positive reaction, yellow colour |
| Klebsiella pneumoniae ATCC 13883 (00097*) | 50-100 | luxuriant | Negative reaction, no colour change | Positive reaction, yellow colour |
| ## Proteus hauseri ATCC 13315 | 50-100 | luxuriant | Negative reaction, no colour change | Positive reaction, yellow colour |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | Negative reaction, no colour change | Positive reaction, yellow colour |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant | Negative reaction, no colour change | Positive reaction, yellow colour |
| Serratia marcescens ATCC 8100 | 50-100 | luxuriant | Negative reaction, no colour change | Positive reaction, yellow colour |
| Shigella flexneri ATCC 12022 (00126*) | 50-100 | luxuriant | Negative reaction, no colour change | Positive reaction, yellow colour |
Key: (*) Corresponding WDCM numbers.
(#) Formerly known as Enterobacter aerogenes
## Formerly known as Proteus vulgaris
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used stated expiry within period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Phenol Red Broth Base |
|---|---|
| SKU | LQ173 |
| Customized Product Available | No |
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