Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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0.1% Peptone
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$39.60
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SKU:
LQ172X3
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Intended use
Recommended as a growth medium and as a base for carbohydrate fermentation media.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone | 1.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ172X3 bottle. In sterile environment, unscrew the bottle. Inoculate the medium with the pre-determined volume of the sample or 50-100 CFU of a known culture (as positive control) and incubate at 35 - 37° C for 24-48 hours.
Principle And Interpretation
Peptone Water, 0.1% is a minimal nutrient media designed to reduce multiplication of microorganisms (1,2). Peptone Water is a non selective media can be utilized as a base for carbohydrate fermentation studies with the addition of sugar and indicators such as bromocresol purple, phenol red or bromothymol blue and for production of indole (3).
Type of specimen
Food samples; Water samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (2).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (1).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further recovery from this enriched medium onto selective media is required.
- Biochemical and serological characterization is required to be carried out from pure isolates for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored atrecommended temperature.
Quality Control
Appearance
Sterile clear 0.1% peptone water in glass bottle.
Colour
Colourless medium
Quantity of medium
30 ml of medium in bottle
pH
6.90-7.30
Sterility Check
Passes release criteria
Cultural characteristics
Cultural characteristics observed after an incubation at 35 - 37°C for 24-48 hours.
| Organism | Inoculum (cfu) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | 50-100 | good |
| Candida albicans ATCC 10231 (00054*) | 50-100 | good |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | 0.1% Peptone |
|---|---|
| SKU | LQ172X3 |
| Customized Product Available | No |
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