Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Mannitol Selenite Broth
As low as
$95.70
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
LQ146
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended use
Recommended for selective enrichment of Salmonellae from clinical specimens.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone | 5.000 |
| Mannitol | 4.000 |
| Sodium phosphate | 10.000 |
| Sodium hydrogen selenite(Sodium biselenite) | 4.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ146 bottle. Inoculate the sample and Incubate at specified temperature and time.
Principle And Interpretation
Selenite-containing media for the enrichment of Salmonella was first described by Guth (1). This medium was further modified by Leifson (2) for the enrichment and isolation of Salmonella from clinical specimens. Mannitol Selenite Broth is a selective enrichment medium, more or less similar to Leifson (2) enrichment medium, described by Hobbs and Allison (3) for the isolation of Salmonella Typhi and Salmonella Paratyphi B from clinical specimens. Mannitol Selenite Broth can also be used for the selective enrichment of Salmonella from water and foodstuffs.
Peptone provides amino acids and other nitrogenous substances to Salmonellae. Mannitol serves as fermentable carbohydrate. Sodium phosphate maintains a uniform pH and also lessens the toxicity of selenite. Do not incubate longer than 24 hours as the inhibitory effect of selenite is reduced after 6-12 hours incubation (4). Subculture broth from the upper third of the broth column to greater or lesser inhibitory selective agars.
Type of specimen
Clinical samples - faeces
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Discard the prepared medium if large amount of selenite is reduced (indicated by red precipitate at the bottom of the tube).
- Do not incubate longer than 24 hours as the inhibitory effect of selenite is reduced after 6 - 12 hours incubation (4).
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label expiry period when stored at within the recommended temperature.
Quality Control
Appearance
Sterile clear Mannitol Selenite Broth in glass bottles
Colour
Light yellow coloured clear solution
Quantity of Medium
10 ml of medium in glass bottle.
Sterility Check
Passes release criteria
pH
6.90-7.30
Cultural Response
Cultural characteristics observed when subcultured on MacConkey Agar (M081), after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Recovery (increase in numbers) | Colour of Colony |
|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | little-none | pink with bile precipitate |
| Salmonella Enteritidis ATCC 13076 | 50-100 | luxuriant | colourless |
| Salmonella Paratyphi B ATCC 8759 | 50-100 | luxuriant | colourless |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | colourless |
Key: (*) corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | Mannitol Selenite Broth |
|---|---|
| SKU | LQ146 |
| Customized Product Available | No |
Write Your Own Review