Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Hartley Broth w/ 0.05% SPS
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$96.80
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Availability: In stock
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SKU:
LQ095
This medium is recommended for the recovery of anaerobic and facultative microorganisms. Sodium polyanetholesulfonate (SPS), an anticoagulant which inhibits the antimicrobial systems of blood, is used widely in blood culture medium and cause an increase in recovery of these organisms. It is available in 20 ml quantity for paediatric use.
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Intended use
For paediatric use. A qualitative test for the recovery of anaerobic and facultative microorganisms. Sterile, in glass bottles.
Composition
| Ingredients | g/L |
|---|---|
| HM hydroysate # | 29.000 |
| Sodium polyanethol sulphonate (SPS) | 0.500 |
| Final pH (at 25°C) | 7.6±0.2 |
# Equivalent to Tryptic digest of heart muscle
**Formula adjusted, standardized to suit performance parameters
Directions:
Label the ready to use blood culture bottle. Do not unscrew cap. remove the top of the screw cap. Disinfect the part of the rubber stopper which is now exposed. Draw patient's blood with the sterile or disposable needle and syringe as explained in specimen collection and disposable column. Transfer the blood sample immediately into the culture bottle by puncturing the rubber stopper with the needle and injecting the blood. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it. Insertion and withdrawal of the needle should be done in a straight line. discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do Not vent the bottle for anaerobic cultures. Incubate at 35±2°C for 18-24 hours and further for seven days.
Principle And Interpretation
Hartley (1) described the value of tryptic digest of muscle for the production of diphtheria toxin. Since then it is used as a general-purpose broth, capable of initiating the growth of demanding (fastidious) organisms from a small inocula. Hartley's Digest Broth can be used for the recovery of fastidious organisms such as Group A, C, G Streptococci and Streptococcus pneumonia from small inocula. Douglas (2) used the same medium to recover Corynebacterium diphtheriae, while Monckton (3) used it in an enrichment medium for C. diphtheriae. Hartley's Digest Broth may be used for cultivation of blood samples, sterility testing, production of diphtheria toxin etc. Hartleys Digest Broth is prepared as per the method described by Cruickshank (4). HM hydrolysate provides nitrogeneous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients. Sodium Polyanethole Sulphonate (SPS) which is an anticoagulant in culture bottles promotes optimal recovery of organisms from blood. It acts to inhibit phagocytosis and to neutralize the antibacterial activity of fresh blood components.
Type of specimen
Clinical sample: Blood
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological testing is required for complete identification.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile clear Hartley Broth w/ 0.05% SPS in glass bottle.
Colour: Light yellow coloured clear solution
Quantity of Medium: 20 ml of medium in glass bottle. (Volume of blood for paediatrics use - 1 to 3 ml)
pH: 7.40-7.80
Sterility Check: Passes release criteria
Cultural response: Cultural characteristics was observed after incubation at 35-37°C for 24-48 hours.
| Organism | Growth |
|---|---|
| Streptococcus pneumoniae ATCC 6303 | Luxuriant |
| Streptococcus pyogenes ATCC 19615 | Luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | Luxuriant |
| Corynebacterium diphtheriae ATCC 11913 | Luxuriant |
Key: (*) Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Hartley Broth w/ 0.05% SPS |
|---|---|
| SKU | LQ095 |
| Customized Product Available | No |
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