Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Bile Broth
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$51.70
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SKU:
LQ079
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Intended Use
Sterile, ready prepared medium for cultivation of members of the Enterobacteriaceae.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone | 20.000 |
| Sodium taurocholate | 5.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
- This product is available in multiple pack sizes of 25 and 50 bottles containing 10ml of sterile medium in each bottle.
- The bottles when supplied are intact. Ensure that all bottles are in upright position and there is no leakage or any manufacturing defect or contamination.
- User may remove the desired number of bottles from the box as per their requirement.
- It should be handled by trained person wearing appropriate personal protective equipment (PPE) and sterile gloves.
- Place the bottles on sterile surfaces such as laminar air flow or sterile working bench.
- Label them accordingly.
- Disinfect the outer surface of cap or closures with suitable disinfectant example 70% IPA.
- Observe aseptic techniques and standard microbiological methods while processing and inoculation of samples or cultures into this media.
- On completion of inoculation tighten the cap and close it carefully.
- Incubate at specified temperature and time or as desired.
- Follow good lab practices for procedures and disposal.
Principle And Interpretation
Enterobacteriaceae inhabit a wide variety of niches that include the human gastrointestinal tract and various environmental niches. When blood samples from a patient with suspected enteric fever is submitted for the widal test, it is useful as a routine to culture the clot after separation of serum (1). Peptone serves as a source of nitrogen, carbon, long chain amio acids and other essential amino acids. Sodium taurocholate would inhibit majority of Gram-positive species. Sodium chloride maintains the isotonicity of the medium. Whereas addition of streptokinase solution causes rapid clot lysis with release of bacteria trapped in the clot (2).
Type of specimen
Clinical samples - Blood clots
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Biochemical characterization is carried out from pure isolates for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature..
Quality Control
Appearance: Sterile clear Bile Broth in glass bottles.
Colour: Light yellow coloured clear solution
Quantity of medium: 10 ml of medium in bottles.
Sterility Check: Passes release criteria
pH: 7.40-7.80
Cultural Response
Cultural characteristics observed after incubation at 35 - 37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited |
Key: (*) Corresponding WDCM numbers, (#) Formerly known as Enterobacter aerogenes
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Bile Broth |
|---|---|
| SKU | LQ079 |
| Customized Product Available | No |
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