HiCombi™ Dual Performance Medium

Label the ready to use LQ033 bottle. Remove the top seal of the cap. Disinfect the part of the rubber stopper which is now exposed. Transfer the sample immediately into the culture bottle by puncturing the rubber stopper with the needle. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it. Insertion and withdrawal of the needle should be done in a straight line. Discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do not vent the bottle for anaerobic cultures. Incubate at 30-35°C for 18-24 hours and further for seven days. Recommended volume of blood to be tested in LQ033: 3-5 ml (For Pediatric use).