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Sterility Kit I (Crimp pack)
Sterility test media prepared in accordance with IP/USP/EP/BP/JP. One Kit contains 2 glass bottles each of 100 ml following media: LQ026 - Sterile Fluid Thioglycollate Medium and LQ027 - Sterile Soyabean Casein Digest Medium. Recommended for injectibles
Composition
Sterile Fluid Thioglycollate Medium M009
| Ingredients | Gms / Litre |
|---|---|
| Pancreatic digest of casein | 17.000 |
| Yeast extract | 5.000 |
| Dextrose | 2.500 |
| Sodium chloride | 5.000 |
| L-Cystine | 0.500 |
| Sodium thioglycollate | 0.500 |
| Resazurin sodium | 0.001 |
| Agar | 0.750 |
Sterile Soyabean Casein DigestMedium M011
| Ingredients | Gms / Litre |
|---|---|
| Pancreatic digest of casein | 17.000 |
| Papaic digest of soyabean meal | 3.000 |
| Dibasic potassium phosphate | 2.500 |
| Dextrose (Glucose) | 2.500 |
| Sodium chloride | 5.000 |
Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use LQ026 bottle. Do not unscrew cap. remove the top of he screw cap. Disinfect the part of the rubber stopper which is now exposed. Draw sample with the sterile or disposable needle and syringe. Transfer the sample immediately into the LQ026 bottle by puncturing the rubber stopper with the injecting needle. Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it (Use sterile venting needle (LA038). Insertion and withdrawal of the needle should be done in a straight line. discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do Not vent the bottle for anaerobic cultures. Incubate at specified temperature for 18-24 hours and further for seven days.
Principle and Interpretation
LQ024 is ready to use sterile prepared media kit contains LQ026 and LQ027 for Sterility Tests in accordance with IP/USP/EP/ BP/JP. These are used for validation of the compliance of pharmacopoeial raw materials, finished products and other articles. The Ready Prepared Media meet the requirements of Growth Promotion Test.
LQ026 :Fluid Thioglycollate Medium is used for rapid cultivation of aerobes as well as anaerobes including microaerophiles.
LQ027: Soybean Casein Digest Medium is recommended for sterility checking and for studying total aerobic microbial count in verification of microbiological testing procedures employed for sterility checking. This medium is a highly nutritious medium used for cultivation of a wide variety of organisms.
Quality Control
Appearance
One kit contains 2 glass bottles each of 100 ml : sterile Fluid Thioglycollate Medium sterile Soyabean Casein Digest Medium
Sterility test
Passes release criteria
Storage and Shelf Life
Store between 15-25°C. Use before expiry date on the label.
| Product Name | Sterility Kit I (Crimp pack) |
|---|---|
| SKU | LQ024 |
| Customized Product Available | No |

