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HiSelective™ E. coli Test Kit

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K023
For rapid detection and confirmation of Escherichia coli from water samples based on enzyme substrate reaction.

Intended Use

HiSelective E. Coli Test Kit is recommended for the rapid detection and confirmation of Escherichia coli based on enzyme-substrate reaction from water samples.

Introduction

The procedures for microbiological examinations of water samples to determine sanitary quality include test for detection and enumeration of indicator organisms. The coliform group of bacteria, especially Escherichia coli is the principle indicator. Various methods for the differentiation of the coliform group are included in standard procedures. Such differentiation however is of limited value in assessing drinking water quality because the presence of any coliform bacteria renders the water potentially unsatisfactory and unsafe. Thus the detection of Escherichia coli in water samples provide qualitative appraising of the sanitary quality of water and the effectiveness of treatment process. Tests for Escherichia coli are applicable for the analysis of drinking water, surface and ground water and waste water. Escherichia coli is a member of the indigenous fecal flora of warm-blooded animals. The occurrence of Escherichia coli is considered a specific indicator of fecal contamination and the possible presence of enteric pathogens.

Directions

  1. Collect 100 ml water to be tested in sterile disposable bottle.
  2. Add entire quantity of medium by swirling to dissolve the powder completely.
  3. After dissolution, incubate the bottle for 24 – 48 hours at 35 – 37°C.
  4. Observe the colour change of the medium from light yellow to blue indicating the presence of Escherichia coli. Coliforms other than Escherichia coli give red colour due to presence of indicator.

Principle and Interpretation

HiSelective E. Coli Test Kit is used for detection and differentiation of Escherichia coli. It is based on Tryptone Bile Agar used for detection of Escherichia coli in foods (1) where recovery of Escherichia coli is faster, more reliable and accurate. The medium contains chromogenic mixture which helps to detect glucuronidase activity of Escherichia coli (2). This specific enzyme differentiates Escherichia coli from other coliforms. Escherichia coli cells split the chromogenic mixture with the help of glucuronidase to give blue colouration to the colonies. Coliforms other than Escherichia coli give red colour due to presence of indicator. Thus, the resulting colour distinction allows simple interpretation of test without further confirmation. Casein enzymic hydrolysate provide the essential growth nutrients to the organisms. Bile salts inhibit gram-positive organisms.

Quality Control

Appearance of Powder :
Light yellow to pink coloured, homogeneous, free flowing powder.

Colour and Clarity :
Light yellow coloured, clear solution.

Cultural Response :
Cultural characteristics after 18-24 hours at 35-37°C

Organisms (ATCC) Growth Colour of Medium
Escherichia coli (25922) luxuriant blue
Enterobacter aerogenes (13048) luxuriant red
S. aureus (25923) fair to good colourless to pink

Storage and Shelf Life

On receipt store between 2-8°C. It has shelf life of 3 years.

More Information
Product Name HiSelective™ E. coli Test Kit
SKU K023
Application/ Industry Water
Product Type Water Testing Kit
Physical Form Not Applicable
Origin Animal
Packaging type PET Bottle
References 1. Anderson J. M. and Baird Parker A.C., 1975, J. Appl. Bact., 39:111.
2.Hansen W. and Yourassawsky E., 1984, J. Clin. Microbiol. 20:1177.
Customized Product Available No
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