HiPer® Dot ELISA Teaching Kit can be used to detect the presence of a test antigen by immobilized antibody bound to the membrane followed by binding of the antigen to the labeled secondary antibody and its detection by using appropriate substrate.
HiPer® Dot ELISA Teaching Kit can be used to detect the presence of a test antigen by immobilized antibody bound to the membrane followed by binding of the antigen to the labeled secondary antibody and its detection by using appropriate substrate. In Dot ELISA, an antibody is immobilized on a membrane and the test antigen is first allowed to
react with immobilized antibody and then to the HRP-labeled antibody. The amount of HRP-labeled antibody
bound is measured by treating the membrane strip with an appropriate chromogenic substrate which is
converted to a coloured precipitate and appears as a dot on the membrane