HiPer® Yeast Transformation Teaching Kit

HiPer® Yeast Transformation Teaching Kit is To prepare Saccharomyces cerevisiae competent cells and transform a yeast plasmid DNA bt Lithium acetate method. Lithium acetate-mediated yeast transformation is the most widely used method for introducing DNA into yeast cells. During yeast transformation the selection of the plasmids are based upon the use of auxotrophic mutant strains which are unable to grow without a specific amino acid. When the yeast strain which contains an auxotrophic mutation (in a specific gene) is transformed with a plasmid containing the wild type copy of that gene, the transformed colonies can be selected by growing the cells on the specific dropout media.