HiPurA® Pre‐ filled Plates for Endotoxin free Plasmid DNA Purification

Intended Use

Recommended for isolation of Plasmid DNA from recombinant E.coli.

Introduction

HiPurA® Pre‐ filled Plates for Endotoxin free Plasmid DNA Purification provides the fastest and easiest way to purify Plasmid DNA for reliable applications in PCR, library screening, sequencing, etc. The DNA purification procedure comprises of three steps viz. adsorption of DNA to the magnetic beads, removal of residual contaminants and elution of pure plasmid DNA. HiPurA® Pre‐ filled Plates for Endotoxin free Plasmid DNA Purification

This kit carries out efficient extraction of Plasmid DNA from recombinant E.coli. Sample is first lysed under the highly denaturing conditions provided by Lysis Solution to inactivate RNases and to ensure isolation of intact Plasmid DNA.

Elution

The yield of DNA depends on the sample type and the number of cells in the sample. A single elution with Elution Solution will provide sufficient DNA to carry out multiple amplification reactions.

Concentration, yield and purity of DNA

Spectrophotometric analysis and agarose gel electrophoresis will reveal the concentration and the purity of the plasmid DNA. Use Elution Buffer (ET) to dilute samples and to calibrate the spectrophotometer, measure the absorbance at 260 nm, 280 nm and 320 nm using a quartz microcuvette. Absorbance readings at 260 nm should fall between 0.1 and 1.0. The 320 nm absorbance is used to correct for background absorbance. An absorbance of 1.0 at 260 nm corresponds to approximately 50 μg/ml of DNA. The A260–A320/A280–A320 ratio should be 1.6‐1.9. Purity is determined by calculating the ratio of absorbance at 260 nm to absorbance at 280 nm. DNA purified by HiPurA® Pre‐ filled Plates for Endotoxin free Plasmid DNA Purification is free of protein and other contaminants that can inhibit PCR or other enzymatic reactions.

Concentration of DNA sample (μg/ml) = 50 x A260 x dilution factor.

Storage

HiPurA® Pre‐ filled Plates for Endotoxin free Plasmid DNA Purification can be stored at room temperature (15‐25°C) for up to 1 year without showing any reduction in performance.