HiPurA® Mag16 HIS-tagged Protein Purification Kit (Magnetic Bead Based)

HiPurA® Mag16 His-tagged Protein Purification Kit (Magnetic Bead Based) is designed for efficient and rapid purification of 6XHis-Tagged proteins using magnetic beads using automated system. These beads have nitrile-triacetic acid (NTA) groups with charged nickel covalently bond to the surface of magnetic beads. These beads having a high affinity for 6xHistagged proteins, under magnetic conditions captures the 6xHis-tagged proteins facilitating the remove of other proteins/ impurities from the crude lysate. The captured his-tagged proteins can be eluted off the magnetic beads by changing the pH or by adding a competitive molecule like imidazole for further use in downstream applications.