HiPurA® DH10B Competent Cells

HiPurA® Competent Cells are specially prepared chemically competent DH10B cells which makes cloning experiments easier. Competent cells are ready to use bacterial cells that contain an altered cell membrane through which a foreign DNA can pass through very easily. HiPurA® Competent Cells are prepared by following a modified protocol of Hanahan (1983, J.Mol.Biol 166:557) and are recommended for cloning of higher molecular weight recombinant plasmids (Greater than 10kb-15kb). DH10B is the most frequently used E.coli strain for routine cloning applications through blue/white screening of colonies on plates containing X-Gal and IPTG as the φ 80lacZΔM15 marker provides αcomplementation of the β-galactosidase gene. A stock pUC19 solution is provided as a control to determine the transformation efficiency. To obtain maximum transformation efficiency, the experimental DNA must be free of phenol, ethanol, protein and detergents. HiPurA® DH10B Competent Cells are engineered for the maintenance of large genomic inserts and complex vectors. These Electroporation Cells ensure High Efficiency Transformation for Large DNA Cloning and Bacterial Artificial Chromosome Mapping.