Hi‐PCR® Cytomegalovirus (CMV) Probe PCR Kit is based on real‐time PCR technology for the detection of Cytomegalovirus (CMV) specific envelope glycoprotein H (UL75) gene. The kit contains primer‐probe mixture specific for detection of CMV DNA. In addition, the kit can identify the DNA extraction efficiency and ensure successful PCR reaction with the help of an endogenous internal control. The kit also provides positive control for the validity of the assay. The assay principle is based on hydrolysis probe chemistry which confers higher specificity and sensitivity.