Hi‐PCR® Acute Fever Panel Kit

Description

Acute fever illness, or acute febrile illness, is characterized by a recent onset of fever accompanied by non‐ specific symptoms such as headache, body rash, and muscle joint pains. The non‐specific presentation of clinical symptoms poses challenge to distinguish the cause of illness based on the clinical history and physical examination alone. This may lead to delay in receiving the appropriate treatment, which may be critical for the patient. A variety of pathogens, including viral, bacterial, and parasitic agents, can cause acute fever. Common pathogens associated with acute fever include Plasmodium (malaria), Dengue virus, Leptospira (leptospirosis), Chikungunya virus, Salmonella (typhoid fever), Scrub typhus (caused by Orientia tsutsugamushi), and Zika virus. Hi‐PCR® Acute Fever Panel Kit is an in‐vitro multiplex probe PCR assay designed for the qualitative detection of Dengue virus, Chikungunya virus, Zika virus, Salmonella spp., Leptospira spp., Plasmodium spp. and Orientia tsutsugamushi in clinical samples. These pathogens are responsible for serious diseases prevalent in many countries, particularly those with tropical climates. Hi‐PCR® Acute Fever Panel Kit detects these pathogens in a single assay with high sensitivity and specificity.

NOTE:

Hi‐PCR® Acute Fever Panel Kit is for in‐vitro use only.

Intended Use:

Hi‐PCR® Acute Fever Panel Kit is designed for sensitive and specific detection of Dengue virus, Chikungunya virus, Zika virus, Salmonella spp., Leptospira spp., Plasmodium spp. and Orientia tsutsugamushi in clinical samples. The Kit is intended for use by qualified clinical laboratory personnel trained in the techniques of real‐time PCR procedures.

Principle

Hi‐PCR® Acute Fever Panel kit, a multiplex Probe PCR Kit, is based on the principle of real‐time PCR. The technique is used to amplify targeted DNA sequence by use of hydrolysis probes that are short oligonucleotides having a fluorescent reporter dye attached to the 5' end and a quencher dye to the 3' end. Hi‐PCR® Acute Fever Panel kit allows simultaneous detection of Dengue virus (DENV), Chikungunya virus (CHIKV), Zika virus (ZIKV), Salmonella spp. (SAL), Leptospira spp. (LEPTO), Plasmodium spp. (PLAS) and Orientia tsutsugamushi (OT) in a two‐tube assay format. Tube 1 includes primers and probes specific for Dengue virus, Chikungunya virus and Zika viruslabelled with the FAM, HEX and Texas Red fluorophores respectively. Tube 2 includes primers and probesspecific for Salmonella spp., Leptospira spp., Plasmodium spp. and Orientia tsutsugamushi labelled with the FAM, JOE, Texas Red and Cy5 fluorophores, respectively. In addition, tube 1 contains an endogenous internal control (IC) amplification system (probe labelled with fluorophore Cy5) to verify efficient total nucleic acid isolation from clinical samples and PCR amplification.