This HiDip slide is recommended for growth of wide variety of microorganisms & cultivation of denitrifying bacteria from soil. TSA-as a general purpose growth medium used for the cultivation of wide variety of microorganisms including bacteria and fungi. Tryptone and Soya peptone supplies the essential nutrients. Asparagine Nitrate Medium - It is formulated as per Subba Rao for soil . Asparagine is source of organic nitrogen and is readily available for microbial energy.