Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiDip™ PA - H₂S Medium
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SKU:
HD012
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Intended Use:
Recommended for detection of coliforms, Salmonella and Citrobacter from water, milk and food.
Composition**
PA Medium
| Ingredients | g/L |
|---|---|
| Peptone | 5.000 |
| Tryptose | 9.830 |
| HM peptone B # | 3.000 |
| Lactose | 7.460 |
| Sodium chloride | 2.460 |
| Dipotassium hydrogen phosphate | 1.350 |
| Potassium dihydrogen phosphate | 1.350 |
| Sodium lauryl sulphate (SLS) | 0.050 |
| Bromo cresol purple | 0.0085 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
#- Equivalent to Beef extract
H2S Medium
| Ingredients | g/L |
|---|---|
| Tryptone## | 15.000 |
| Yeast extract | 5.000 |
| Sodium hydrosulphite | 0.500 |
| Dextrose | 5.500 |
| Resazurin | 0.001 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.9±0.2 |
**Formula adjusted, standardized to suit performance parameters
## Equivalent to Casein enzymic hydrolysate
Directions
- Surfaces: Loosen cap and remove HiDip™ slide from container taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slides and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for 15-20 seconds. Take care not to smudge agar over the test surface. Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temprature.
- Liquids: Loosen cap and remove the HiDip™ slide from container. Check for dehydration or contamination.Dip slide into test fluid for upto 15-20 seconds so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain. Tab it gently to remove excess fluid from surface. Return the slide to the container and close tightly. Incubate in an upright position at indicated temprature. Label the container for sample number, source, date and time etc. for reference.
Principle And Interpretation
PA Agar is used for the detection of coliforms bacteria in milk, food and water from treatment plants or distribution systems. This medium has been included as a tentative standard in the "Standard Methods for the Examination of Water and Wasterwater", APHA.
H2S medium is recommended for simultaneous detection of Salmonella, Vibrio, Citrobacter species and Escherichia coli from milk food and water samples. Presence of growth with or without change of colour of medium indicates microbial contamination of specimen. Incubate slides at 35 - 37°C for 18-24 hours to observe cultural characteristics.
Type of specimen
Food and dairy samples, Water samples
Specimen Collection and Handling:
Refer Directions.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
PA Medium
- PA test is only a presumptive test for the presence of coliforms. Confirmation of these results must be achieved by using a medium like Brilliant Green Bile Broth (M121).
H2S Medium
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
The HiDip™ slide containing a combination of sterile PA Medium and H2S medium on separate individual surfaces.
Colour
Colour of PA Medium
Greyish purple coloured medium
Colour of H2S Medium
Greenish blue coloured medium
Quantity of medium
2.5 ml per surface
Sterility Check :
Passes release criteria
pH of PA Medium
pH Range: 6.60-7.00
pH of H2S Medium
pH Range: 7.70-8.10
Cultural Response
Cultural characteristics observed after incubation at 35 - 37°C for 18-48 hours
PA Medium
| Organism | Growth | Color of the colony |
|---|---|---|
| Escherichia coli ATCC 25922 | good-luxuriant | Colourless |
| # Klebsiella aerogenes ATCC 13048 (00175*) | good-luxuriant | Colourless |
| Enterococcus faecalis ATCC 29212 | inhibited | - |
| Klebsiella pneumoniae ATCC 13883 | good-luxuriant | Colourless to off white |
| Salmonella Typhimurium ATCC 14028 | good-luxuriant | Colourless |
H2S Medium
| Organism | Growth | Color of the colony |
|---|---|---|
| Citrobacter freundii ATCC 8090 | good-luxuriant | Blue w/black centre |
| Escherichia coli ATCC 25922 (00013*) | Good-luxuriant | Blue |
| Klebsiella pneumoniae ATCC 13883 | Good-luxuriant | Purple |
| Salmonella Enteritidis ATCC 13076 (00030*) | Good-luxuriant | Blue w/black centre |
| Salmonella Typhimurium ATCC 14028 (00031*) | Good-luxuriant | Blue w/black centre |
| Vibrio cholerae ATCC 15748 | Good-luxuriant | Bluish purple |
Key: (*) Corresponding WDCM numbers. (#) Formerly known as Enterobacter aerogenes
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
Used HiDip™ slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap.
| Product Name | HiDip™ PA - H₂S Medium |
|---|---|
| SKU | HD012 |
| Customized Product Available | No |
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