Agar Agar, Type I

SKU:
GRM666
CAS Number : 9002-18-0
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Agar Agar, Type I is recommended for preperation of culture media for microbiological analysis.
CAS Number : 9002-18-0


Product Identifier

Product Name Agar Agar, Type I
SKU GRM666

Directions:

Agar Agar Type I is produced for use in bacteriological routine laboratory work, plant tissue culture media, pharmaceutical preparations, where clarity, compatibility are not of prime importance. When suspended in cold water, it swells but does not dissolve. However, it readily dissolves in boiling water and solubility is facilitated by soaking the powder in cold water.

Safety Information

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Safety guidelines may be referred in individual safety data sheets.

Technical Specification

Limitations
  1. It is biological origin product since variation in colour of powder and clarity may observed.
  2. Each lot of the product has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's requirement.
  3. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium prepared by the product.
Performance and Evaluation Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature
Appearance Cream coloured powder homogeneous free flowing powder.
Solubility Freely soluble in hot water at temperatures above 85°C. Insoluble in cold water.
Clarity A firm solid, clear to slightly opalescent gel is formed at a concentration of 1.5% at 38-41 °C.
pH pH of 1.5% w/v aqueous solution at 25 °C 6.0 - 7.0
Dye Diffusion Agar dye diffusion :- 18-20mm
Identification test (As per method specified in USP 2022)
  • A: Infrared absorption
  • B: Iodine TS colours some of the fragments of Agar bluish black, with some areas reddish to violet.
  • C: Agar forms a clear liquid, that congeals at 30-39°C to form a firm resilient gel, which does not liquefy below 80°C.
Microbial Load - Bacterial Count <= 1000 CFU/gram by plate method, when incubated at 30-35°C for not less than 3 days
Microbial Load - Yeast & mould Count <= 100 CFU/gram by plate method, when incubated at 20-25°C for not less than 5 days.
Test for pathogens
  1. Escherichia coli- Absent/gram of sample
  2. Salmonella species- Absent/10 gram of sample
  3. Pseudomonas aeruginosa- Absent/gram of sample
  4. Staphylococcus aureus- Absent/gram of sample
  5. Candida albicans- Absent/gram of sample
  6. Clostridia- Absent/gram of sample
Test for Water absorption (As per method specified in USP 2022) NMT 75 ml of water is absorbed by 5.0 g of agar
Limit of Gelatin (As per method specified in USP 2022) No yellow precipitate is formed.
Limit of Foreign Starch (As per method specified in USP 2022) The sample solution does not, upon cooling, produce a blue colour upon the addition of iodine TS.
Growth Promotion Test (As per method specified in USP 2022) Cultural response observed after an incubation at 35-37°C for 18-24 hours by preparing Nutrient Agar (M001) using Agar Agar, Type I as an ingredient.
Gelling temperature 38-41°C
Melting Range >=85°C
Water (KF) <=20%
Total Nitrogen <= 0.125%
Arsenic (As) <= 3 ppm
Lead(Pb) <= 10 ppm
Acid- Insoluble Ash (On dry-Weight basis) <=0.5%
Total Ash (On dry-weight basis) <=6.5%
Foreign organic matter <=1.0%
Limit of Foreign insoluble matter <=15 mg in 7.5 gm of Agar

Cultural Response

Organism Growth
Escherichia coli ATCC 25922 (WDCM00013) Luxuriant
Pseudomonas aeruginosa ATCC 27853 (WDCM 00025) Luxuriant
Staphylococcus aureus subsp. aureus ATCC 25923(WDCM 00034) Luxuriant
Salmonella enterica subsp. enterica Typhi ATCC 6539 Luxuriant
Streptococcus pyogenes ATCC 19615 Luxuriant
Salmonella enterica subsp. enterica Enteritidis ATCC 13076 (WDCM 00030) Luxuriant
Salmonella enterica subsp. enterica Typhimurium ATCC 14028 (WDCM00031) Luxuriant
Yersinia enterocolitica subsp. enterocolitica ATCC 9610 (WDCM 00038) Luxuriant
Yersinia enterocolitica subsp. enterocolitica ATCC 23715 (WDCM 00160) Luxuriant
CAS Number : 9002-18-0
More Information
Product Name Agar Agar, Type I
SKU GRM666
CAS No 9002-18-0
EC No. 232-658-1
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