This medium is recommended by APHA for estimating the heterotrophic plate count by the pour plate, spread plate or membrane filter procedure. A low nutrient medium like R-2A Agar incubated for longer incubation periods allows these stressed organisms to grow well.
1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Wastewater,Wastewater, 20th Ed., American Public Health Association, Washington, D.C.2.Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of Foods,5th Ed., American Public Health Association, Washington, D.C.3.Reasoner D. J. and Geldreich E. E., 1985, Appl. Environ. Microbiol., 49:1.4.Collins V. J. and Willoughby J. G., 1962, Arch. Microbiol., 43:294.5.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.6.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.66