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Minerals Modified Glutamate Agar Base, Granulated (Twin Pack)

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GM643I
Recommended for the enumeration of Escherichia coli from meat and meat products. The composition and performance criteria of this medium are as per the specifications laid down in ISO 6391:1997.

Composition

Ingredients Gms / Litre
Lactose 10.000
Dipotassium phosphate 0.900
Sodium formate 0.250
L-Cystine 0.020
L-Aspartic acid 0.024
L-Arginine 0.020
Thiamine 0.001
Nicotinic acid 0.001
Pantothenic acid 0.001
Ferric ammonium citrate 0.010
Calcium chloride, 2H2O 0.010
Magnesium sulphate, 7H2O 0.100
Agar 15.000
Sodium glutamate 6.350
Final pH (at 25°C) 6.7±0.2

Formula adjusted, standardized to suit performance parameters

Directions

Suspend 26.29 grams (the equivalent weight of dehydrated granulated medium) of Part A and 6.35 grams of Part B in 1000 ml distilled water containing 2.5 grams ammonium chloride. Heat if necessary to dissolve the medium completely. Cool to 45-50°C. Sterilize by autoclaving at 10 lbs (115°C) for 10 minutes. Mix well and pour into sterile Petri plates.

Principle And Interpretation

Folpmers (1) described a glutamic acid based chemically defined medium for the enumeration of coliform bacteria from water and wastewater. This glutamate-containing medium was later modified by Gray (2), by the addition of lactose, which gave less false positive results when compared to MacConkey Broth.

Mineral Modified Glutamate Agar Base is recommended for the enumeration of Escherichia coli from meat and meat products by colony count at 44°C by membrane filtration technique. Anderson and Baird-Parker (3) described a direct plate method for the rapid enumeration of E. coli in foods and this method was modified by a resuscitation procedure using Mineral Modified Glutamate Agar with successful recovery of damaged cells from frozen, dried, heat processed or low pH foods.

This media contain a variety of nutrients including salts, amino acids and vitamins. Lactose is the fermentable carbohydrate. Because of the nutrients, this media is superior for enumerating coliforms in water and wastewater as it satisfies most of the nutritional requirements of coliforms.

Quality Control

Appearance
Part A: Off-white to light yellow coloured granular medium Part B: White to cream homogenous free flowing powder

Gelling
Firm, comparable with 1.5% Agar gel

Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates

Reaction

Reaction of the medium (2.634% w/v Part A + 0.635% w/v Part B + 0.25%ammonium chloride) is at 25°C. pH : 6.7±0.2

pH

6.50-6.90

Cultural Response

Cultural characteristics observed after an incubation at 44°C for 24 hours.

Organism Inoculum (CFU) Growth Recovery
Escherichia coli ATCC 25922 50-100 luxuriant >=50%

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

More Information
Product Name Minerals Modified Glutamate Agar Base, Granulated (Twin Pack)
SKU GM643I
Product Type Granulated
Physical Form Granular
Origin Animal
Packaging type HDPE
References 1. Folpmers T., 1948, J. Microbiol. Serol., 14.58-64
2.Gray R. D., 1959, J. Hyg. Camb. 57:249.
3.Gray R. D., 1964, J. Hyg. Camb., 62:495.
4.P. H. L. S. Standing Committee on the Bacteriological Examination of Water Supplies, 1968, J. Hyg. Camb. 65:67.
5.Abbiss J. S., Wilson J. M., Blood R. M. and Jarvis B., 1981, J. Appl. Bacteriol., 51:121.
6.Department of Environment, Health and Social Security and P. H. L. S., 1982, The Bacteriological Examination of WaterSupplies, Report on Public Health and Medical Subjects No. 71, H.M.S.O., London, England7.Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.American Public Health Association, Washington, D.C.
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