Pikovskaya’s Broth (Medium), Granulated is recommended for detection of phosphate solubilizing microorganisms. Phosphate as calcium phosphate is present in the medium. The growth obtained in this medium may be detected for phosphate solubilization by subculturing or spot inoculation on Pikovskayas agar (M520). Phopshate solubilization is indicated as clearance around growth or colony.