Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Semisolid RV Medium Base, Granulated
As low as
$212.30
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
GM1428
For isolation of Salmonella species from food stuffs and other materials based on selective motility.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Semisolid RV Medium Base, granulated is used for the isolation of Salmonella from food stuffs and other materials based on selective motility.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Tryptose | 4.600 |
| Casein enzymic hydrolysate | 4.600 |
| Sodium chloride | 7.340 |
| Magnesium chloride, anhydrous | 10.930 |
| Malachite green oxalate | 0.037 |
| Agar | 2.700 |
| Final pH (at 25°C) | 5.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 15.10 grams in 500 ml distilled water. Heat with stirring to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and aseptically add 1 vial of IMRV/RV Selective Supplement (FD193). Mix well and pour into sterile Petri plates.
Note: The motility of Salmonellas can be drastically reduced when the agar surface becomes too dry. Hence the plates should be well dried before use. If visible moisture occurs on the lid of the plates or the surface of agar, it must be removed. While incubation, incubate the plates aerobically in an upright position for no longer than 24 hours at 42°C.
Principle And Interpretation
Semisolid RV Medium Base is based on the formulation described by DeSmedt et al (1) for the detection of motile Salmonella species from food and environmental specimens. This medium detects more Salmonella positive samples than the routinely used enrichment procedures (2, 3, 4).
Tryptose, casein enzymic hydrolysate provides the nitrogenous and carbonaceous substances and other essential growth nutrients. The motility of other microorganisms is largely inhibited by the selective agents (magnesium chloride, malachite green and novobiocin).
The working of medium is based on the ability of Salmonella species to migrate in the selective medium competing with the other motile organisms, thus producing opaque halos of growth. The motile bacteria will show a halo or zone of growth originating from inoculation spot.
Quality Control
Appearance
Light yellow to light blue coloured granular medium
Gelling
Semisolid, comparable with 0.27% Agar gel.
Colour and Clarity of prepared medium
Blue coloured clear to slightly opalescent semisolid gel forms in Petri plates.
Reaction
Reaction of 3.02% w/v aqueous solution at 25°C. pH : 5.4±0.2
pH
5.20-5.60
Cultural Response
Cultural characteristics observed after an incubation at 42-43°C for 18-24 hours with added IMRV/RV Selective Supplement (FD193)when one drop of culture is inoculated in the centre of the medium plate. (Motility is checked by inoculating a drop of culture in the centre of the medium plate).
| Organism | Inoculum (CFU) | Growth | Motility |
|---|---|---|---|
| Citrobacter freundii ATCC 8090 | >=10³ | inhibited | - |
| Pseudomonas aeruginosa ATCC 9027 | >=10³ | inhibited | - |
| Salmonella Enteritidis ATCC 13076 | 50-100 | good-luxuriant | Positive reaction, colourless to light pink zone |
| Salmonella Typhimurium ATCC 14028 | 50-100 | good-luxuriant | Positive reaction, colourless to light pink zone |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Semisolid RV Medium Base, Granulated |
|---|---|
| SKU | GM1428 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. De Smedt J.M., Balderdijk R., Rappold H. and Lautenschlaeger D., 1986, J. Food Prot., 49:510. |
| Customized Product Available | No |
Write Your Own Review
Semisolid RV HiVeg™ Medium Base
As low as
$269.50
Semisolid Nutrient Agar
As low as
$248.60
Semisolid Rappaport Vassiliadis Medium Base, Modified
As low as
$185.90
Semisolid IMRV Medium Base
As low as
$269.50
Semisolid RV Medium Base
As low as
$269.50
Modified Semisolid RV Medium Base
As low as
$248.60
Semisolid RV Medium w/0.9% Agar
As low as
$184.80
IMRV, RV Selective Supplement
As low as
$88.00
Upsell Products
Modified Semisolid RV Medium Base, Granulated
As low as
$272.80