Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Modified EC Broth Base, Granulated
As low as
$216.70
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
GM1285
For isolation of Escherichia coli O157 : H7.
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Composition**
| Ingredients | Gms/Litre |
|---|---|
| Peptic digest of animal tissue | 20.000 |
| Sodium chloride | 5.000 |
| Lactose | 5.000 |
| Bile salts mixture | 1.120 |
| Dipotassium hydrogen phosphate | 4.000 |
| Potassium dihydrogen phosphate | 1.500 |
| Final pH (at 25°C) | 6.9±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 18.31 grams in 500 ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45 – 50°C and aseptically add rehydrated contents of 2 vials of Novobiocin Supplement(FD096) . Mix well and dispense in sterile tubes or flasks as desired.
Principle And Interpretation
EC Medium was originally developed by Hajna and Perry (1) for improved detection of coliforms. The current formulation is a combination of lactose broth, bile salts mixture and buffering agents.
Peptic digest of animal tissue serves as a rich source of nitrogen, vitamins and amino acids. Lactose provides the carbon source for lactose fermenting microorganisms. The addition of bile salts mixture inhibits the growth of gram positive bacteria particularly bacilli and faecal streptococci. Sodium chloride maintains the osmotic balance of the medium.Potassium salts buffers the medium. The addition of novobiocin inhibits gram positive bacteria.
Quality Control
Appearance
Cream to yellow coloured granular medium
Colour and Clarity of Prepared Medium
Light amber clear
Reaction
Reaction of 1.83% w/v aqueous solution at 25°C. pH : 6.9±0.2
pH
6.70-7.10
Cultural Response
Cultural characteristics observed with added Novobiocin Supplement (FD096) after an incubation at 35-37°C for 18-24 hours.
Cultural Response
| Organism | Inoculum(CFU) | Growth |
|---|---|---|
| Escherichia coli O157:H7 | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant |
| Enterococcus faecalis ATCC 29212 | >=10³ | inhibited |
| Staphylococcus aureus ATCC 25923 | >=10³ | inhibited |
| Bacillus subtilis ATCC 6633 | >=10³ | inhibited |
Storage and Shelf Life
Store below 30°C in tightly closed container and prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Modified EC Broth Base, Granulated |
|---|---|
| SKU | GM1285 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Hajna A.A., a. Perry C.A., 1943, Am. J. Pbl. Hlth. 33:550-556. |
| Customized Product Available | No |
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