Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Standard Nutrient Broth No. 1, Granulated
As low as
$255.20
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
GM1224
For cultivation of fastidious bacteria.
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Standard Nutrient Broth No. 1, granulated is used for the cultivation of fastidious bacteria
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone, special | 15.000 |
| Yeast extract | 3.000 |
| Sodium chloride | 6.000 |
| Dextrose | 1.000 |
| Final pH (at 25°C) | 7.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Nutrient media are basic culture media used for maintaining microorganisms (1), for purity checking prior to biochemical or serological testing. The Peptone special, incorporated in this broth is an enzymatic protein digest of animal tissue especially adapted for the preparation of media for culturing fastidious bacteria. The peptone and yeast extract provides nitrogenous compounds, carbon, sulphur, trace ingredients. Sodium chloride maintains the osmotic equilibrium.
Addition of different biological fluids such as horse or sheep blood, serum, egg yolk etc. makes it suitable for the cultivation of related fastidious organisms (2).
Quality Control
Appearance
Cream to yellow coloured granular medium
Colour and Clarity of prepared medium
Light amber coloured clear solution in tubes
Reaction
Reaction of 2.5% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH
7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant |
| Erysipelothrix rhusiopathiae ATCC 19414 | 50-100 | good-luxuriant |
| Listeria monocytogenes ATCC 19111 | 50-100 | good-luxuriant |
| Staphylococcus aureus ATCC 25923 | 50-100 | good-luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
| Shigella flexneri ATCC 12022 | 50-100 | good-luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Standard Nutrient Broth No. 1, Granulated |
|---|---|
| SKU | GM1224 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Lapage S., Shelton J. and Mitchell T., 1970, Methods in Microbiology , Norris J. and Ribbons D.(Eds.), Vol. 3A., AcademicPress, London. |
| Customized Product Available | No |
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