YEPG Growth Medium

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SKU:
G061
YEPG Growth Medium is recommended for the growth of Saccharomyces cerevisiae


Intended Use

YEPG Growth Medium is recommended for the growth of Saccharomyces cerevisiae.

Composition

Ingredients Grams/Litre
Peptone 20.00
Yeast extract 10.00

Final pH (at 25°C) 6.5 ± 0.2

Formula adjusted, standardized to suit performance parameters

Directions

Suspend 30 grams in 1000 ml distilled water containing 38 ml glycerol. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Principle and Interpretation

YEPG Growth Medium is recommended for the growth of Saccharomyces cerevisiae. Respiratory deficient mutants will not grow on YEPG. Glycerol is a non-fermentable carbon source.

Quality Control

Appearance of Powder: Cream to yellow coloured, homogeneous, free flowing powder.

Colour and Clarity: Light yellow coloured, clear solution without any precipitate.

Reaction: Reaction of 3.0% w/v aqueous solution containing 3.8% v/v glycerol is pH 6.5 ± 0.2 at 25°C.

Cultural Response

Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.

Organisms (ATCC) Growth
Saccharomyces cerevisiae ATCC9763 good-luxuriant

Storage and Shelf Life

Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.

More Information
Product Name YEPG Growth Medium
SKU G061
Customized Product Available No
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