Super Broth

Composition**

Ingredients

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 60 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Principle And Interpretation

Escherichia coli is a bacterium that is commonly found in the gut of humans and warm-blooded animals. Most strains of E. coli are harmless. Some strains however, such as Enterohaemorrhagic E. coli (EHEC) can cause severe foodborne disease. Super Broth has a formulation slightly different from that described by Atlas (1) and it is used for the mass cultivation of E. coli

Casein enzymic hydrolysate and yeast extract provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Sodium chloride maintains osmotic equilibrium. Super Broth is nutritionally rich hence other organisms can also grow in it easily.

Quality Control

Appearance Cream to yellow homogeneous free flowing powder

Colour and Clarity of prepared medium Light yellow coloured clear solution without any precipitate

Cultural Response M1316: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Reference

1. Atlas R.M., 2004, Handbook of Microbiological Media, Parks L.C. (Ed.), CRC Press, Inc