Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiEncap™ Super Broth
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SKU:
EC1316CCL
For mass cultivation of Escherichia coli.
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Intended Use
Recommended for mass cultivation of Escherichia coli.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 35.000 |
| Yeast extract | 20.000 |
| Sodium chloride | 5.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 15 gms of medium. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Escherichia coli is a bacterium that is commonly found in the gut of humans and warm-blooded animals. Most strains of E. coli are harmless. Some strains however, such as Enterohaemorrhagic E. coli (EHEC) can cause severe foodborne disease. Super Broth has a formulation slightly different from that described by Atlas (1) and it is used for the mass cultivation of E. coli.
Tryptone and yeast extract provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Sodium chloride maintains osmotic equilibrium. Super Broth is nutritionally rich hence other organisms can also grow in it easily.
Type of specimen
Isolated Microorganism of Escherichia coli.
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- 1. Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Gelatin capsule containing cream to yellow coloured granular media
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate
Quantity: Each capsule containing 15 grams of medium sufficient for 250 ml media
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Cultural Response
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | good-luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiEncap™ Super Broth |
|---|---|
| SKU | EC1316CCL |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1. Atlas R.M., 2004, Handbook of Microbiological Media, Parks L.C. (Ed.), CRC Press, Inc |
| Customized Product Available | No |
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