Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Chlorella Broth Base w/o Dextrose and Citrate
As low as
$57.20
Availability: Out of stock
Availability: In stock
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SKU:
M769
Used for cultivation of chlorella.
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Blue Green Algae#CC293D
Intended Use
Recommended for for cultivation of Chlorella species.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Cupric sulphate | 0.0078 |
| Sodium molybdate | 0.050 |
| Zinc sulphate | 0.220 |
| Boric acid | 0.00028 |
| Manganese sulphate | 0.0014 |
| Ferrous sulphate | 0.0015 |
| Potassium sulphate | 0.217 |
| Magnesium sulphate | 2.400 |
| Potassium dihydrogen phosphate | 2.450 |
| Potassium nitrate | 2.500 |
| Final pH (at 25°C) | 4.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 7.6 grams in 900 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Add aseptically 100 ml of separately sterilized solution of 10 gm of dextrose and 32 mg of potassium citrate. Mix well and dispense into sterile tubes or flasks as desired.
Principle And Interpretation
Chlorella is a genus of single-celled green algae, belonging to the phylum Chlorophyta. Chlorella Broth has originally formulated by Shrift (5) and further modified for cultivation and maintenance of Chlorella.
All algae utilize inorganic phosphates and sulphates. There is a fairly high requirement of molybdate as a trace metal in nitrogen fixation. Calcium, magnesium, potassium and probably sodium are required by algae. Most algae grow poorly on agar and it is best to let them become established in liquid culture before adapting them to the more rigorous conditions of an agar slant.
Chlorella Broth Base w/o Dextrose and Citrate is the same as Chlorella Broth except that the citrate and dextrose have been omitted from the medium. This media supplies the necessary nutrients for the rapid growth of Chlorella species. Chlorella being photosynthetic green algae should be cultivated in the presence of light. Bright diffused light, fluorescent light and sunlight are satisfactory sources of light for the growth of Chlorella. The inoculated tubes/flasks should be incubated in the presence of light at 25-27°C for a week to permit good growth and pigmentation (4). Chlorella cultures can be maintained at room temperature for 2-3 months without subculturing.
Type of specimen
Water samples
Specimen Collection and Handling
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards(1). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- The inoculated tubes/flasks should be incubated in the presence of light at 25-27°C for a week to permit good growth and pigmentation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
White to cream homogeneous free flowing powder
Colour and Clarity of prepared medium
Colourless clear solution in tubes
Reaction
Reaction of 0.76% w/v aqueous solution at 25°C. pH: 4.5±0.2
pH
4.30-4.70
Cultural Response
Cultural characteristics observed in presence of light,after an incubation at 25-27°C for 1 week.
| Organism | Growth |
|---|---|
| Chlorella vulgaris ATCC 9765 |
good-luxuriant |
| Euglena gracilis ATCC 12716 |
good-luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Chlorella Broth Base w/o Dextrose and Citrate |
|---|---|
| SKU | M769 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1. Shrift, 1954, Am. J. Botany, 41:223. 2.Norris J.R. & Ribbons D.W. (ed.), 1963, Methods in Microbiology, Volume 3B, Academic press, London, pg. 269. |
| Customized Product Available | No |
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Chlorella Broth
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