Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Pikovskaya’s Agar
As low as
$51.70
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
M520
For detection of phosphate-solubilizing soil microorganisms.
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Culture Media#CC293D
Pikovskayas Agar is recommended for detection of phosphate-solubilizing soil microorganisms.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Yeast extract | 0.500 |
| Dextrose | 10.000 |
| Calcium phosphate | 5.000 |
| Ammonium sulphate | 0.500 |
| Potassium chloride | 0.200 |
| Magnesium sulphate | 0.100 |
| Manganese sulphate | 0.0001 |
| Ferrous sulphate | 0.0001 |
| Agar | 15.000 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31.3 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Phosphate exists in both organic as well as inorganic forms in soil. Organic matter derived from dead and decaying plant debris is rich in organic sources of phosphorus. However, plants are able to utilize phosphorus from soil only in the free available form. Soil phosphates are rendered available either by plant roots or by soil microorganisms. Therefore, phosphate-dissolving soil organisms play a part in correcting phosphorus deficiency of crop plants (1). Pikovskayas Agar was modified by Sundara Rao and Sinha (2) for detection of phosphate-solubilizing bacteria from soil.
Yeast extract in the medium provides nitrogen and other nutrients necessary to support bacterial growth. Dextrose acts as an energy source. Different salts and yeast extract supports the growth of organisms. Phosphate-solubilizing bacteria will grow on this medium and form a clear zone around the colony, formed due to phosphate solubilization in the vicinity of the colony.
Quality Control
Appearance: White to light yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: White with flocculant precipitate opaque gel forms in Petri plates
Cultural Response: M520: Cultural characteristics observed after an incubation at 35-37°C for 48 hours.
| Organism | Growth | Phosphate solubilization |
|---|---|---|
| *Aspergillus brasiliensis ATCC 16404 | luxuriant | positive reaction, clear zone surrounding the colony |
| Bacillus subtilis ATCC 6633 | good | moderate clear zone surrounding the colony |
| Pencillium notatum ATCC 10108 | luxuriant | positive reaction, clear zone surrounding the colony |
| Pseudomonas aeruginosa ATCC 27853 | luxuriant | positive reaction, clear zone surrounding the colony |
*Key: Formerly known as Aspergillus niger ATCC 16404
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label..
Reference
- Subba Rao N. S., 1977, Soil Microorganisms and Plant Growth, Oxford and IBH Publishing Co., New Delhi.
- Sundara Rao W. V. B. and Sinha M. K., 1963, Ind. J., Agric. Sci., 33:272.
| Product Name | Pikovskaya’s Agar |
|---|---|
| SKU | M520 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal Free (Microbial) |
| Packaging type | HDPE |
| References | 1. Subba Rao N. S., 1977, Soil Microorganisms and Plant Growth, Oxford and IBH Publishing Co., New Delhi. 2.Sundara Rao W. V. B. and Sinha M. K., 1963, Ind. J., Agric. Sci., 33:272. |
| Customized Product Available | No |
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