Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Citrate Agar
As low as
$44.00
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SKU:
M728
Used for cultivation of iron bacteria from soil samples.
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Iron Bacteria#CC293D
Intended use
Recommended for cultivation of iron bacteria from soil samples.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Ammonium sulphate | 0.500 |
| Sodium nitrate | 0.500 |
| Magnesium sulphate | 0.500 |
| Dipotassium phosphate | 0.500 |
| Calcium chloride | 0.200 |
| Ferric ammonium citrate | 10.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.7±0.1 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 27.2 grams in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
The iron bacteria oxidize ferrous iron to ferric state, which precipitate as ferric hydroxide around cells. These bacteria are usually non-filamentous and spherical or rod shaped. Certain algae also transform ferrous salts to ferric state and deposit the precipitation around the colonies. The ferric hydroxide deposits give a brown or rust red colour to these organisms. Citrate Agar is recommended by Subba Rao (5) for the isolation and detection of iron bacteria. A modification of the original formulation of Subba Rao is recommended by APHA (1) for the isolation of heterotrophic iron-precipitating bacteria (2). Dipotassium phosphate provides buffering to the medium. Magnesium sulphate, ammonium sulphate and calcium chloride are sources of ions that stimulate metabolism. Ferric ammonium citrate is used as a source of carbon and sodium nitrate acts as a source of nitrogen.
Type of specimen
Soil samples; Water samples
Specimen Collection and Handling
For soil samples, follow appropriate techniques for sample collection and processing as per guidelines (5).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(1)
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- This medium is general purpose medium for soil bacteria and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to greenish yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 2.72% w/v aqueous solution at 25°C. pH : 6.7±0.1
pH
6.60-6.80
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for upto 7 days.
| Organism | Growth |
|---|---|
| Escherichia coli ATCC 25922 (00013*) | inhibited |
| Sphaerotilus natans ATCC 13338 | good-luxuriant |
Key:*- Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Citrate Agar |
|---|---|
| SKU | M728 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1. Subba Rao N. S., 1977, Soil Microorganisms and Plant Growth, Oxfordand IBH Publishing Co., New Delhi. 2.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water andWastewater, 23rd ed., APHA, Washington, D.C. 3.Clark F. M., Scott R. M. and Bone E., 1967, Heterotrophic, iron-precipitating bacteria, J. Am. Water Works Assoc., 59: 1036. 4.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition. 5.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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