Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Ringer Salt Solution, Granulated
As low as
$40.70
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
GM525
An isotonic diluent for food, milk and dairy products during microbiological examination.
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Total Bacterial Count- Colony Count Method#CC293D
Intended Use
Recommended as an isotonic diluent for food, milk and dairy products during microbiological examinations.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Sodium chloride | 8.500 |
| Potassium chloride | 0.200 |
| Calcium chloride anhydrous | 0.200 |
| Sodium bicarbonate | 0.010 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters.
Directions
Suspend 8.91 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Any diluent used in microbiological examination should be isotonic with the cells to be suspended. It should also preferably contain a buffer and certain ions necessary for the optimal maintenance of cells. Ringer Salt Solution Powder is recommended as an isotonic diluent for microbiological examination of foods (1,2).
Ringer Salt Solution is isotonic with bacteria and thus prevents them from being subjected to osmotic stress when they are removed from their customary environment. It is physiologically superior to physiological saline for sensitive organisms. Ringer Salt Solution is used as an isotonic diluting fluid and suspending fluid which preserves the cells in their original condition. The salts in the medium balances the osmotic equilibrium of the medium, thereby protecting the organisms from osmotic stress caused due to change in environment.
Type of specimen
Food samples; Water samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (3).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- It is physiologically superior to physiological saline for sensitive organisms, however the viability has to be checked.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: White to cream coloured granular medium
Colour and Clarity of prepared medium: Colourless clear solution without any precipitate
Reaction: Reaction of 0.891% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response
Satisfactory results are obtained when used as a diluent during bacteriological examination of foods, milk, dairy products as well as for serial dilutions of pure cultures of bacteria.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | Ringer Salt Solution, Granulated |
|---|---|
| SKU | GM525 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1. Finegold S. M and Baron E. J, 1986, Bailey and Scotts Diagnostic Microbiology, 7th Edition, The C.V. Mosby Co. 2.Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), 1996,Mackie and McCartney, Practical MedicalMicrobiology, 14th Edition, Churchill Livingstone |
| Customized Product Available | No |
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