Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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APT Agar, Granulated
As low as
$174.90
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
GM226
Recommended for the cultivation of heterofermentative lactic acid bacteria and other organisms requiring high thiamine content.
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Total Bacterial Count- Colony Count Method#CC293D
APT Agar, granulated is recommended for the cultivation of heterofermentative lactic acid bacteria and other organisms requiring high thiamine content.
Composition
| Ingredients | Gms/Litre |
|---|---|
| Casein enzymic hydrolysate | 12.500 |
| Yeast extract | 7.500 |
| Dextrose | 10.000 |
| Sodium citrate | 5.000 |
| Sodium chloride | 5.000 |
| Dipotassium phosphate | 5.000 |
| Magnesium sulphate | 0.800 |
| Manganese chloride | 0.140 |
| Ferrous sulphate | 0.040 |
| Polysorbate 80 | 0.200 |
| Thiamine hydrochloride | 0.001 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.7±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 61.18 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. AVOID EXCESSIVE HEATING. Cool to 45-50°C. Mix well and pour into sterile Petri plates or tubes or as desired.
Principle And Interpretation
APT (All purpose Tween 80) Agar is formulated as per Evans and Niven (1) for cultivation and maintenance of Lactobacilli. This medium is also used in the microbiological assay of thiamine. Lactobacillus forms a major part of lactic acid bacteria group which are abundant in nature. They convert lactose and other sugars to lactic acid and therefore are named as Lactobacillus. They are responsible for spoilage of foods like meat, dairy etc. However APT Agar can also be used for cultivation of heterofermentative lactic acid bacteria requiring high thiamine content (2). APT Agar is also used as a maintenance medium since it preserves the viability and sensitivity of Lactobacillus viridescens ATCC 12706.
APT Agar contains casein enzymic hydrolysate, which acts as a source of carbon, nitrogen, vitamins and minerals. Yeast extract provides vitamin and B-complex nutrients, which is required for the growth of bacteria. Dextrose is the carbohydrate source. Manganese chloride, magnesium sulphate and ferrous sulphate provide ions used in replication by lactobacilli. Polysorbate 80 is a source of fatty acids required by lactobacilli.
Quality Control
Appearance
Cream to yellow coloured granular medium
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 6.12% w/v aqueous solution at 25°C. pH: 6.7±0.2
pH
6.50-6.90
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Lactobacillus acidophilus ATCC 4356 | 50-100 | good-luxuriant | >=50% |
| Lactobacillus viridescens ATCC 12706 | 50-100 | good-luxuriant | >=50% |
| Leuconostoc mesenteroides ATCC 12291 | 50-100 | good-luxuriant | >=50% |
| Lactobacillus casei ATCC 9595 | 50-100 | good-luxuriant | >=50% |
| Lactobacillus plantarum ATCC 8014 | 50-100 | good-luxuriant | >=50% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on label.
| Product Name | APT Agar, Granulated |
|---|---|
| SKU | GM226 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Evans and Niven, 1951, J. Bact., 62:599. 2.Downes F. P. and Ito K. (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th ed.,APHA, Washington D.C. |
| Customized Product Available | No |
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