Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
Lactobacillus MRS Agar (MRS agar), Granulated
As low as
$214.50
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
GM641I
Your enquiry has been submitted
Please login to Post an Enquiry.
Acid Producing Microorganisms#CC293D
Composition
ISO 13721:1995(E) specification
| Ingredients | g / L |
|---|---|
| Meat extract | 8.000 |
| Peptone | 10.000 |
| Yeast extract | 5.000 |
| Triammonium citrate | 2.000 |
| Sodium acetate | 5.000 |
| Magnesium sulphate, heptahydrate | 0.200 |
| Manganese sulphate, tetrahydrate | 0.050 |
| Dipotassium hydrogen phosphate | 2.000 |
| Glucose, anhydrous | 20.000 |
| Sorbitan monooleate (Tween 80) | 1.000 |
| Agar | 12.0-18.0 |
| pH after sterilization (at 25°C) | 5.7 |
Lactobacillus MRS Agar, Granulated® GM6411
| Ingredients | g / L |
|---|---|
| HM Peptone B# | 8.000 |
| Peptone | 10.000 |
| Yeast extract | 5.000 |
| Ammonium citrate | 2.000 |
| Sodium acetate | 5.000 |
| Magnesium sulphate, heptahydrate | 0.200 |
| Manganese sulphate, tetrahydrate | 0.050 |
| Dipotassium phosphate | 2.000 |
| Dextrose (Glucose) | 20.000 |
| Polysorbate 80 (Tween 80) | 1.000 |
| Agar | 12.000 |
| Final pH (at 25°C) | 5.7±0.2 |
**Formula adjusted, standardized to suit performance parameters # - Equivalent to Beef extract
Directions
Suspend 65.13 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Lactobacilli MRS medium is based on the formulation of deMan, Rogosa and Sharpe (1) with slight modification. It supports luxuriant growth of all Lactobacilli (1), dairy products (2), foods (3) and other sources (4). Lactobacillus MRS Agar is recommended by ISO Committee (5). Peptone and HM Peptone B supplies nitrogenous and carbonaceous compounds, long chain amino acids and other essential growth nutrients. Yeast extract provides vitamin B complex and glucose is the fermentable carbohydrate and energy source. Polysorbate 80 supplies fatty acids required for the metabolism of Lactobacilli. Sodium acetate and ammonium citrate inhibit Streptococci, moulds and many other microorganisms. Phosphates provide good buffering action in the media. Lactobacilli are microaerophillic and generally require layer plates for aerobic cultivation on solid media. When the medium is set, another layer of un-inoculated MRS Agar is poured over the surface to produce a layer plate (4). Lactobacilli isolated on MRS Agar should be further confirmed biochemically.
Type of specimen
Water samples; Food and dairy samples
Specimen Collection and Handling
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (6). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (7,8,9). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical tests are required for complete identification.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance:
Cream to light yellow coloured granular medium
Gelling:
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium:
Medium to dark amber coloured slightly opalescent gel forms in Petri plates
Reaction:
Reaction of 6.51% w/v aqueous solution at 25°C. pH : 5.7±0.2
pH:
5.50-5.90
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours (longer if neccesary) (with 5% CO2)
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Lactobacillus acidophilus ATCC 4356 | 50-100 | luxuriant | >=50% |
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant | >=50% |
| Lactobacillus fermentum ATCC 9338 | 50-100 | luxuriant | >=50% |
| ^Lactiplantibacillus plantarum ATCC 8014 | 50-100 | luxuriant | >=50% |
| Lactococcus lactis subsp. lactis ATCC 19435 | 50-100 | luxuriant | >=50% |
| Lactococcus sakei ATCC 15521 | 50-100 | luxuriant | >=50% |
| Pediococcus damnosus ATCC 29358 | 50-100 | luxuriant | >=50% |
| Pediococcus pentosaceus ATCC 33316 | 50-100 | luxuriant | >=50% |
| Bifidobacterium bifidum* ATCC 11863 | 50-100 | luxuriant | >=50% |
| Escherichia coli ATCC 25922 | 50-100 | none-poor | <=10% |
Key: (*) -Growth under anaerobic conditions for 72 hours ^ Formerly known as Lactobacillus plantarum
Storage and Shelf Life
Store dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
| Product Name | Lactobacillus MRS Agar (MRS agar), Granulated |
|---|---|
| SKU | GM641I |
| Customized Product Available | No |
Write Your Own Review