Streptomyces Medium

Streptomyces Medium is recommended for the cultivation and maintenance of Streptomyces kanamyceticus.

Composition**

Final pH (at 25°C): 7.0±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 36.34 grams of dehydrated medium in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.

Principle And Interpretation

Streptomycetes i.e. aerobic Actinomycetes usually inhabit soil. In humans, the infections are limited to actinomycotic mycetoma. Streptomycetes are considered to be a treasure for production of antibiotics. Streptomyces kanamyceticus is especially useful for the production of kanamycin A, B and also acetyltransferases (2). Streptomyces Medium is used for the cultivation and maintenance of S. kanamyceticus. This species is of significant use as kanamycin antibiotic is prepared (extracted) using these fungi.

Though many carbon sources have been used for the growth of S. kanamyceticus, glucose is found to be the most suitable carbon source for the production of antibiotic from it (1). L-Glutamic acid is the nitrogen source. The salts provide essential ions for the growth of S. kanamyceticus.

Quality Control

Appearance: Off-white to light yellow homogeneous free flowing powder

Gelling: Firm, comparable with 2.5% Agar gel

Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates

Reaction: Reaction of 3.63% w/v aqueous solution at 25°C. pH : 7.0±0.2

pH: 6.80-7.20

Cultural Response

M1330: Cultural characteristics observed after an incubation at 25-30°C for upto 7 days.

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Reference

1. Rodney A, Shukla A and Majumdar. African Journal of Biotechnology Vol 4 (9). 909-910, 2005.
2. Balows A., Truper H. G., Dworkin M., Harder W., Schleifer K. H., (Ed.), The Prokaryotes, 1992, 2nd Edition, Vol. III, Springer-Verlag.