Solid medium recommended for preparing and isoalting competent cells prior to transformation, Super Optimal Broth (SOB medium) Growth Medium was first formulated by Douglas Hanahan in 1983 as a nutritionally enriched growth media for bacteria, especially Escherichia coli,used in the preparation and transformation of chemicallycompetent cells
1. Hanahan, D., 198 3.J. Mol. Biol.166:557. 2.Sambrook J., E. F. Fritsch, and T. Maniatis. 1989.Molecular cloning: A laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.