Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiEncap™ YT Agar
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SKU:
EC1369D
For growth of Escherichia coli K12 strains used in preparation of phage and plasmid DNA according to Miller.
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Intended Use
Recommended for growth of Escherichia coli K12 strains used in preparation of phage and plasmid DNA according to Miller.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 8.000 |
| Yeast extract | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 10.000 |
Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 14 gms of medium. Suspend 1 capsule in 500 ml (2 capsules in 1000 ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates or dispense as desired.
Principle And Interpretation
YT Agar is used for the growth of Escherichia coli K12 strain used in preparation of phase and plasmid DNA according to Miller (1).
Tryptone and yeast extract provides sources of nitrogen and growth factors which allow the bacteria to recover from the stress of transformation and grow well. Sodium chloride provide essential ion.
Type of specimen
Isolated Recombinant strain of Escherichia coli
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Gelatin capsule containing cream to yellow coloured granular media
Gelling
Firm, comparable with 1.0% Agar gel.
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent gel forms in Petri plates.
Quantity
Each capsule contains 14 grams of medium sufficient for 500 ml media
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
Organism Cultural Response
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant | >=70% |
| Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | luxuriant | >=70% |
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiEncap™ YT Agar |
|---|---|
| SKU | EC1369D |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1.H. Miller Meth Enzymol; 152 ,145, (1987) |
| Customized Product Available | No |
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