Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiEncap™ YT Broth (HiEncap™ 2 XT Broth)
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$0.00
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Availability: In stock
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SKU:
EC1251CCL
For the cultivation of recombinant strains of Escherichia coli.
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Intended Use
Recommended for the cultivation of recombinant strains of Escherichia coli.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 16.000 |
| Yeast extract | 10.000 |
| Sodium chloride | 5.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 7.75 grams of medium. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) distilled or purified water. Heat to boiling to dissolve the medium completely. Mix well and dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
YT Broth is recommended for use in the cultivation of recombinant strains of Escherichia coli (1,5,6). It is also used in culture of E. coli strains for propagation of M 13 bacteriophages (1,2,6). These media contain tryptone and yeast extract, which supply nitrogenous compounds, vitamin B complex and other essential nutrients and co-factors necessary for the luxuriant growth of recombinant E. coli and allows the bacteria to recover from the stress of transformation and grow well. Sodium chloride helps in maintaining isotonic conditions in the medium.
Type of Specimen
Isolated Recombinant strain of Escherichia coli
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Gelatin capsule containing light yellow to beige coloured granular media
Quantity
Each capsule contains 7.75 grams of medium sufficient for 250ml media.
Color and Clarity of Prepared Medium
Light amber coloured clear solution in tubes.
Reaction
Reaction of 3.1% w/v aqueous solution at 25°C. pH: 7.0±0.2
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | good-luxuriant |
| Escherichia coli ATCC 53868 | 50-100 | good-luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | HiEncap™ YT Broth (HiEncap™ 2 XT Broth) |
|---|---|
| SKU | EC1251CCL |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1. Miller H., 1987, Meth. Enzymol; 152, 145. 2. Ausubel F. M., Brent R., Kingston R. E., Moore B. D., Seidman J. G., Smith J. A. and Strohl K., 1994, Current Protocols in Molecular Biology, Vol. I, Current Protocols, New York, N.Y. 3. Davis L. G., Dibner M. D., Battey J. F., 1986, Basic Methods in Molecular Biology, Elsevier, New York, N.Y. 4. Sambrook J., Fritsch E. E. and Maniatis T., 1989, Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. |
| Customized Product Available | No |
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