Ni-NTA Agarose Resin

Ni-NTA Agarose Resin uses nitrilotriacetic acid (NTA), a tetradentate chelating ligand, in a highly cross-linked 6% agarose matrix. NTA binds Ni2+ ions by four coordination sites. His-tagged proteins expressed in E. coli can be purified and detected easily because the histidine residues bind metal ions like nickel immobilized on a column matrix under specific buffer conditions and bound proteins are eluted out by changing the pH or by adding a competitive molecule like imidazole. Ni-NTA Agarose Resin has an orientative binding capacity of up to 50 mg/ml gel.