Protein G Agarose Resin

HiMedia's Protein G Agarose Resin consists of rProtein G covalently bound to crosslinked agarose beads and has a very high affinity for IgG (immunoglobulins). rProtein G is a genetically engineered recombinant protein which contains three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure the maximum specific IgG binding. The recombinant Protein G has been covalently immobilized onto 4% cross-linked agarose. Immobilized Protein G is ideal for polyclonal IgG purification from mouse, human, cow, goat and sheep serum, including human IgG3 and mouse IgG1 isotypes. When a suspension (e.g. serum, ascites fluid, tissue culture supernatant) containing mixture of substances along with IgG are loaded on the column, the IgG binds to Protein G and is recovered by elution. This resin is used for batch or column purifications of classes, subclasses and fragments of immunoglobulins from serum, ascites fluid, tissue culture supernatant. It provides a very stable bond that can greatly minimize leakage of the Protein G allowing for reuse of the affinity resin in several purification steps. This product is supplied as a slurry suspension of Protein G Agarose Resin in 20% ethanol. 1 ml resin corresponds to 2 ml of 50 % (v/v) Protein G Agarose slurry suspension. Protein G Agarose Resin provides an alternative binding profile for immunoglobulin subclasses with low affinity for Protein A. This resin facilitates antibody purification during affinity chromatography and molecular protein research testing of various species and IgG types.