HiMedia's Coating buffer is phosphate buffer designed to use in any ELISA format. It helps to stabilize the coating antigen or antibody added to the wells and maximize the adsorption of the protein to polystyrene plates. By stabilizing the adsorbed protein the antigenic regions are preserved allowing greater binding reactivity and enhanced specific signal. Coating Buffer ensures optimal pH and ionic strength for the passive adsorption of proteins onto hydrophobic plastic surfaces. It facilitates antigen immobilization during ELISA plate preparation and molecular diagnostic assay development for consistent experimental results.
Coating is the first step in any ELISA. Coating means the immobilization of antigen, antibodies or any other compound on the well surface. HiMedia’s Coating buffer is phosphate buffer designed to use in any ELISA format. It helps to stabilize the coating antigen or antibody added to the wells and maximize the adsorption of the protein to polystyrene plates. By stabilizing the adsorbed protein the antigenic regions are preserved allowing greater binding reactivity and enhanced specific signal.