Neutralization Solution (HN3)

Neutralization Solution (HN3) is a buffer solution allows the renaturation of DNA during the isolation of plasmid from recombinant E.coli cells. This is extensively used in molecular biology experiments. This buffer of potassium acetate is added to neutralize the strong alkaline conditions of the lysis buffer. During this neutralization procedure the large chromosomal DNA is captured in the precipitate, whereas the small plasmid DNA remains in solution. Neutralization Solution (HN3) is a concentrated acidic salt mixture used to restore neutral pH and precipitate genomic DNA and proteins after alkaline treatment. This Neutralization Solution ensures rapid PH Adjustment and Salt Precipitation for any professional Molecular Plasmid Recovery Lab.