Hippurate Hydrolysis Test is used for detection of hippurate hydrolyzing bacteria, mainly Streptococcal species. Group B streptococci (Streptococcus agalactiae) and some enterococci can hydrolyze 1% aqueous sodium hippurate to produce glycine and sodium benzoate. Glycine is deaminated by the oxidizing agent ninhydrin which gets reduced and becomes purple. Enterococcus faecalis ATCC 29212: negative precipitate if any, dissolves on shaking ; Streptococcus agalactiae ATCC 4768 positive brown flocculant