Lead Acetate Paper Strips are used for detection of hydrogen sulphide production by microorganisms. The lead acetate procedure is more sensitive than any other method for detecting H2S production. It detects even traces of H2S.
H2S is a colourless gas which on contact with lead acetate produces lead sulphide, a black precipitate, indicated by a visible
black coloured reaction on the Lead acetate paper strip. Escherichia coli ATCC 25922 negativereaction, no blackening ; Salmonella Enteritidis ATCC 13076 positive reaction, blackening of the lower portion of the strip.