X+V Factor disc is used for the presumptive identification of Haemophilus species on the basis of their requirements for X or V factors or both.Both X and V factors are growth factors that are essential for certain organisms like Haemophilus species and also enhance growth of organisms like Neisseria species. X+V factor discs are the sterile filter paper discs impregnated with growth factors x & V which are used for differentiating Haemophilus species in conjuction of X factor & V factor discs.The test organism requiring X factor alone, grows only in the vicinities of X and X+V factor discs. Those which require V factor alone grow in the vicinities of V and X+V factor discs. If both X and V factors are required, then the organism will grow only in the vicinity of the X+V factor discs.Bordetella pertussis ATCC 8467 :Positive with X factor ; Haemophilus influenzae ATCC 35056: Negative