Leibovitz’s L-15 Medium w/ 2mM L-Glutamine

Leibovitz’s Medium was specifically designed to grow cells in a CO2 free atmosphere. The standard sodium bicarbonate/ CO2 buffering system is replaced by combination of free basic amino acids, phosphate buffers and higher levels of galactose and sodium pyruvate. The medium can be used to grow human tumor cells and embryonic cells and also established cell lines like HeLa and Hep-2. The medium is frequently used in diagnostic virology where tissue cell lines or strains need to be grown in closed systems. Leibovitz’s medium obviates the need of frequent medium change. AL247A contains 2mM L-glutamine.