CIN Agar is selective differential medium for isolation of Yersinia enterocolitica from food and clinical specimens. Contains three antibiotics: Cefsulodin - inhibits Pseudomonas, Irgasan (triclosan) - inhibits gram-positives, Novobiocin - inhibits Proteus. Y. enterocolitica produces bull's-eye colonies (dark red center with transparent border) due to mannitol fermentation. Cold enrichment (4°C for 3 weeks) prior to plating enhances recovery from food. Incubate 24-48 hours at 28-30°C (not 35-37°C). Used for outbreak investigations, pork testing. The composition and performance criteria of this medium are as per the specifications laid down in ISO 10273:2017(E)