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Carbonate Buffer for Coating
Coating isthe firststep in any ELISA. Coating meansthe immobilization of antigen, antibodies or any other compound on the well surface. It is the process where a suitably diluted antigen or antibody is incubated until adsorbed to the surface of the microtiter plate. Adsorption occurs passively as the result of hydrophobic interactions between the amino acids side chains on the antibody or antigen used for coating, and the plastic surface. It is dependent upon time, temperature, and the pH of the coating buffer, as well as the concentration of the coating agent.
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HiMedia’s Carbonate Buffer for Coating is a carbonate?based buffer designed to use in any ELISA format for proteins and antibody coating on polystyrene plate. It helps to immobilize and stabilize the coating antigen or antibody added to the wells to maximize the adsorption of the protein to polystyrene plates. By stabilizing the adsorbed protein, the antigenic regions are preserved allowing greater binding reactivity and enhanced specific signal.
| Product Name | Carbonate Buffer for Coating |
|---|---|
| SKU | ML224 |
| Customized Product Available | No |
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